Investigation of Morphology of Novel Anthraquinone-Containing Polymeric Wound Healers
By adding anthraquinone to polyvinyl alcohol (PVA) polymer, nanocomposite mat production will be provided by nanotechnological electrospinning technique. Material properties will be determined by morphological (Scanning Electron Microscopy-SEM) and mechanical (TENSILE) analysis of the produced nanocomposites.
It is thought that nanofiber wound dressing tapes containing original anthraquinone analogues will provide a new perspective to studies in the health sector and biomaterials in the future.
1. Introduction
The skin is the organ with the largest surface area, which consists of three main components: epidermis, dermis and subcutaneous fat layer, and protects our body against external factors. While the outer epidermis protects the body against damage such as burns and injuries, the inner dermis protects the body against trauma.
The main structural component of the underlying dermis is collagen, which is located in fibrils (Lee, 2000; Kazaroglu, N.M., 2009). Wound healing means restoring the integrity of tissue or organ after injury. Injured tissues are partially or completely repaired (Topalan,
M., & Aktaş, Ş., 2010).
In this study, chloroanthraquinone doped PVA nanofiber membranes were produced by electrospinning technique. The produced membranes were characterized. It is aimed to use the obtained composite membranes as a wound dressing that will provide ideal properties.
2. Material and Method
2.1. Material
In this study, 85,000-124,000 g/mol Sigma/Aldrich (Germany) brand PVA was used. Greaseproof paper was preferred in the electrospinning stage.
2.2. Method
2.2.1. Production Of Wound Healing Nanofiber Membranes By Electrospinning Method.
The electrospinning parameters required for the fabrication of wound healing nanofiber membrane are shown in Table 2.1.
The nanofiber membranes to be obtained will be placed in a vacuum oven and dried at 50oC for one day. The schematic representation of the production of anthraquinone-doped nanotechnological wound dressing tape by electrospinning is given in Figure 2.1.
2.2.2. Characterization Studies of Nanocomposite Mats
Morphological Analysis
Field Emission Gun Scanning Electron Microscopy (FEGSEM)
Nanofiber composites will be coated with gold-palladium material under argon gas for 40 seconds. After the coating process, the samples will be viewed with high and low vacuum detectors on the Quanta brand FEI FEG450 model FESGEM device at x12000 magnification.
Mechanical Analysis
Tensile Test
Tensile testing of nanofiber composites in accordance with ASTM D882-10 standards will be carried out in a DEVOTRANS brand DVT UZM K3 model tensile-compression test device.
5 mm2/min. to the samples under 500 N load. The mechanical properties will be determined by adjusting the pulling speed and 10 mm jaw spacing.
3. Result and Discussion
Morphological (SEM) Analysis
Nanofiber formation was observed in all samples. As the anthraquinone analog is added, the fibers are more tightly packed. While the orientation of the fibers occurred more, the highest strength in the study was realized in 10% PVA-8% Unique anthraquinone derivative
sample (Ozkan and Sahin TR2016/19610; Bulus et al., 2020).
SEM images of nanofiber membranes are shown in Figure 3.1., Figure 3.2., Figure 3.3., Figure 3.4., Figure 3.5. Nanofiber size distribution range of nanofiber membranes is shown in Table 3.1.
4. Conclusion
In this study, anthraquinone-doped PVA nanofiber membranes were produced by electrospinning technique. According to the characterization studies, the fiber diameters are in the range of 170-230 nm according to their morphological characterization, and
they have the thinnest fibers in this study.
It was determined that the thinnest fibers belonged to 10% PVA- 8% Anthraquinone compound. The resulting composites are intended to be an ideal band-aid.
Acknowledgements
This work was financially supported by the Scientific Research Projects Coordination Unit of Istanbul University- Cerrahpasa (Project number: BEK-2017-26753) for supplying the equipment and material.
We would like to thank ArelPOTKAM for his contributions to the determination of the mechanical properties of nanofiber membranes in production and material supply.
Kaynaklar
1. Lee, K.H., Tissue-engineered human living skin substitutes: Development and clinical application, Yonsei Medical Journal, 2000, 41, 6, 774-779.
2. Kazaroğlu, N.M. Yara örtüleri için alternatif doku iskeleleri: in vitro çalışmalar, 2009.
3. Topalan, M., & Aktaş, Ş. (2010). Güncel Yönleriyle Kronik Yara.
4. Ozkok, F., Sahin, Y.M. Biyoaktif Antrakinon Analogları ve Bunların Sentezine Yönelik Metot. TR2016/19610.
5. Buluş, E., Buluş, G. S., Akkaş, M., Cetin, T., Yaman, E., & Altındal, T. (2020). Nanotechnological Wound Healing
Bandage Production from Polymer Solutions Containing Tea Tree Oil, Echinacea, Spider Web and Aloe Vera. Journal Of Materials And Electronıc Devices, 6(1), 19-23.
Res. Assist. Dr. Funda Özkok - Chemistry Departmant
Engineering Faculty
İstanbul Cerrahpaşa University
Prof. Dr. Nihal Onul - Chemistry Departmant
Engineering Faculty
İstanbul Cerrahpaşa University
Assoc. Prof. Yeşim Müge Şahin - ArelPOTKAM
Departmant of Biomedical Engineering
Faculty of Engineering and Architecture
İstanbul Arel University
Instructor Erdi Buluş - ArelPOTKAM
Department of Transportation Services Civil Aviation
Cabin Services Program
Vocational School
İstanbul Arel University
Gülseren Sakarya Buluş - Uzman Hemşire
Istanbul Provincial Health Directorate
Department of Engineering Management
Graduate School of Education
Bahçeşehir University
